Abstract
This research was undertaken to test the in vitro anti-inflammatory action of 5,7,4'-trimethoxy-4-phenylcoumarin and 5,7-dimethoxy-4-phenylcoumarin produced by Streptomyces aureofaciens CMUAc130. The effects of the two coumarins were investigated on the formation of NO, PGE2, and TNF-alpha and also on inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-induced murine macrophage RAW 264.7 cells. The data obtained were consistent with the modulation of iNOS enzyme expression. A similar effect was also observed when LPS-induced PGE2 release and COX-2 expression were tested. The inhibitory effects were shown in concentration-dependent manners. The 5,7,4'-Trimethoxy-4-phenylcoumarin and 5,7-dimethoxy-4-phenylcoumarin also mildly but significantly reduced the formation of TNF-alpha.
Publication types
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Research Support, Non-U.S. Gov't
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Retracted Publication
MeSH terms
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Cell Line
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Coumarins / pharmacology*
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Cyclooxygenase 2 / immunology
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Cyclooxygenase 2 Inhibitors / pharmacology
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Dinoprostone / biosynthesis
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Dinoprostone / immunology
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Lipopolysaccharides / antagonists & inhibitors
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Lipopolysaccharides / pharmacology
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Macrophages / drug effects*
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Macrophages / immunology
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Mice
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Nitric Oxide / biosynthesis
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Nitric Oxide / immunology
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Nitric Oxide Synthase Type II / antagonists & inhibitors
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Nitric Oxide Synthase Type II / immunology
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Streptomyces aureofaciens / chemistry*
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Streptomyces aureofaciens / immunology
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Tumor Necrosis Factor-alpha / antagonists & inhibitors
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Tumor Necrosis Factor-alpha / immunology
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Coumarins
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Cyclooxygenase 2 Inhibitors
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Lipopolysaccharides
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Tumor Necrosis Factor-alpha
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Nitric Oxide
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Nitric Oxide Synthase Type II
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Ptgs2 protein, mouse
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Cyclooxygenase 2
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Dinoprostone