Abstract
The interactions between malignant cells and the microenvironment of the local host tissue play a critical role in tumor growth, metastasis and their response to treatment modalities. We investigated the roles of smooth muscle calponin (Cnn1, also called calponin h1 or basic calponin) in the development of tumor vascul ature in vivo by analyzing mutant mice lacking the Cnn1 gene. Here we show that loss of Cnn1 in host mural cells prevents maturation of tumor vasculature. In vitro studies showed that platelet-derived growth factor B-induced vascular smooth muscle migration was downregulated by the Cnn1-deficiency, and forced expression of Cnn1 restored migration. Moreover, destruction of established tumor mass by treatment with an antivascular endothelial growth factor antibody was markedly enhanced in Cnn1-deficient mice. These data, coupled with the knowledge that structural fragility of normal blood vessels is caused by loss of the Cnn1 gene, suggest that Cnn1 plays an important role in the maturation of blood vessels, and may have implications for therapeutic strategies targeting tumor vasculature for treatment of human cancers.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / analysis
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Animals
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Antibodies / pharmacology
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Antigens, CD34 / analysis
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Apoptosis / drug effects
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Becaplermin
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Blood Vessels / drug effects
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Blood Vessels / metabolism*
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Blood Vessels / pathology
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Calcium-Binding Proteins / genetics
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Calcium-Binding Proteins / physiology*
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Calponins
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Carcinoma, Lewis Lung / blood supply*
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Carcinoma, Lewis Lung / genetics
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Carcinoma, Lewis Lung / pathology
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Cell Line, Tumor
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Cell Movement / drug effects
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Cells, Cultured
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Immunohistochemistry
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Male
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Melanoma, Experimental / blood supply*
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Melanoma, Experimental / genetics
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Melanoma, Experimental / pathology
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Mice, Knockout
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Mice, Nude
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Microfilament Proteins / genetics
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Microfilament Proteins / physiology*
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Muscle, Smooth / chemistry
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Platelet Endothelial Cell Adhesion Molecule-1 / analysis
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Platelet-Derived Growth Factor / pharmacology
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Proto-Oncogene Proteins c-sis
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Vascular Endothelial Growth Factor A / immunology
Substances
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Actins
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Antibodies
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Antigens, CD34
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Calcium-Binding Proteins
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Microfilament Proteins
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Platelet Endothelial Cell Adhesion Molecule-1
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Platelet-Derived Growth Factor
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Proto-Oncogene Proteins c-sis
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Vascular Endothelial Growth Factor A
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Becaplermin