Abstract
Different domains of a novel full-length var gene (termed 732var) isolated from a placenta of a malaria-infected woman were expressed in Escherichia coli as recombinant proteins and analysed biochemically and immunologically. Two of these, the Duffy binding-like (DBL)-3gamma domain and the cysteine-rich interdomain region (CIDR)-1alpha were able to bind chondroitin sulfate A and CD36, respectively. The DBL-3gamma domain was investigated in a previous study and confirmed here to exhibit anti-disease characteristics related to pregnancy-associated malaria. Mothers with high anti-DBL-3gamma antibody levels were protected from placental infection. The novel finding in this study is that babies born to mothers carrying anti-CIDR-1alpha antibodies had a delayed time to the first infection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Animals
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Antigens, Protozoan / chemistry
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Antigens, Protozoan / genetics
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Antigens, Protozoan / immunology*
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Antigens, Protozoan / metabolism
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CD36 Antigens / metabolism
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Chondroitin Sulfates / metabolism
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Duffy Blood-Group System
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Female
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Humans
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Immunoglobulin G / blood
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Immunoglobulin G / immunology*
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Infant, Newborn
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Malaria, Falciparum / immunology*
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Malaria, Falciparum / parasitology
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Malaria, Falciparum / prevention & control
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Placenta
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Plasmodium falciparum / immunology*
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Pregnancy
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Pregnancy Complications, Parasitic / immunology*
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Pregnancy Complications, Parasitic / parasitology
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Pregnancy Complications, Parasitic / prevention & control
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Protozoan Proteins / chemistry
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Protozoan Proteins / genetics
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Protozoan Proteins / immunology*
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Protozoan Proteins / metabolism
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Receptors, Cell Surface / chemistry
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / immunology*
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Receptors, Cell Surface / metabolism
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology*
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Recombinant Proteins / metabolism
Substances
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Antigens, Protozoan
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CD36 Antigens
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Duffy Blood-Group System
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Duffy antigen binding protein, Plasmodium
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Immunoglobulin G
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Protozoan Proteins
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Receptors, Cell Surface
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Recombinant Proteins
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Chondroitin Sulfates