HIV type 1 variants with nevirapine resistance mutations are rarely detected in antiretroviral drug-naive African women with subtypes A, C, and D

AIDS Res Hum Retroviruses. 2007 Jun;23(6):764-8. doi: 10.1089/aid.2006.0272.

Abstract

K103N is frequently detected in HIV-infected women after single dose (SD) nevirapine (NVP). K103N-containing variants were detected more frequently by the ViroSeq HIV-1 Genotyping System in women with subtype C (69.2%) than subtypes A (19.4%, p < 0.0001) or D (36.1%, p < 0.0001). K103N-containing variants were also detected more frequently and at higher levels in women with subtype C by the LigAmp assay. In this report, we analyzed samples collected prior to or within hours after SD NVP administration from antiretroviral drug-naive African women with subtypes A, C, and D. Only 1/254 samples had an NVP resistance mutation detected with the ViroSeq system, and only 4/236 samples had K103N detected at < 0.5% with the LigAmp assay [2/110 (1.8%) with subtype A, 1/46 (2.2%) with subtype C, and 1/80 (1.3%) with subtype D] (p = 0.92). We did not detect significant differences in the pre-NVP frequency of NVP resistance mutations or the pre-NVP levels of K103N-containing variants in women with subtypes A, C, and D that explain the dramatic subtype-based differences in emergence of HIV-1 variants with these mutations after SD NVP exposure.

Publication types

  • Clinical Trial
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Africa
  • Anti-HIV Agents / pharmacology*
  • Black People
  • DNA Mutational Analysis
  • Drug Administration Schedule
  • Drug Resistance, Viral / genetics*
  • Female
  • Genotype
  • HIV Infections* / drug therapy
  • HIV Infections* / genetics
  • HIV-1 / classification
  • HIV-1 / drug effects
  • HIV-1 / genetics*
  • Humans
  • Infectious Disease Transmission, Vertical / prevention & control
  • Nevirapine / pharmacology*
  • Polymorphism, Single Nucleotide / genetics*
  • Pregnancy
  • Pregnancy Complications, Infectious

Substances

  • Anti-HIV Agents
  • Nevirapine