The connections between the host and 5-hydroxytryptamine-containing neurons grafted to the spinal cord have been analysed using electron microscopic immunohistochemistry. Adult rats with 5,7-dihydroxytryptamine lesions of the brain and spinal cord received implants of embryonic medullary raphé neurons at three sites in the spinal cord. Eight to 10 months after grafting, the transplanted 5-hydroxytryptamine-positive neurons had formed extensive and complex contacts with spines, dendrites, perikarya and vesicle-containing structures in both the dorsal and ventral horns. Reinnervation of laminae IV-VI was less rich. In the graft itself, connections were also made between non-immunoreactive varicosities and 5-hydroxytryptamine-containing dendrites, and somata, but the exact origin of the afferents was not determined. Outside the implant site, no obvious synaptic junctions onto grafted 5-hydroxytryptamine-immunoreactive boutons were obvious, although labelled and unlabelled varicosities were often in close apposition. Synaptic junctions in the dorsal horn were predominantly symmetric, with the presynaptic varicosity containing mostly small agranular vesicles. By contrast, in the ventral horn most junctions were asymmetric, while the presynaptic element contained both small agranular and large dense-core vesicles. The results demonstrate that the types of synaptic contacts formed between the grafted 5-hydroxytryptamine neurons and the host spinal cord are remarkably similar to those found in intact spinal cord. In addition, the division of morphological differences that exists between 5-hydroxytryptamine-containing boutons in the normal dorsal vs ventral horns is also apparent in the transplanted animals. Finally, there appear to be present several anatomical substrates for the regulation by the host of 5-hydroxytryptamine output from the grafted neurons.