A reciprocal tensin-3-cten switch mediates EGF-driven mammary cell migration

Nat Cell Biol. 2007 Aug;9(8):961-9. doi: 10.1038/ncb1622. Epub 2007 Jul 22.

Abstract

Cell migration driven by the epidermal growth factor receptor (EGFR) propels morphogenesis and involves reorganization of the actin cytoskeleton. Although de novo transcription precedes migration, transcript identity remains largely unknown. Through their actin-binding domains, tensins link the cytoskeleton to integrin-based adhesion sites. Here we report that EGF downregulates tensin-3 expression, and concomitantly upregulates cten, a tensin family member that lacks the actin-binding domain. Knockdown of cten or tensin-3, respectively, impairs or enhances mammary cell migration. Furthermore, cten displaces tensin-3 from the cytoplasmic tail of integrin beta1, thereby instigating actin fibre disassembly. In invasive breast cancer, cten expression correlates not only with high EGFR and HER2, but also with metastasis to lymph nodes. Moreover, treatment of inflammatory breast cancer patients with an EGFR/HER2 dual-specificity kinase inhibitor significantly downregulated cten expression. In conclusion, a transcriptional tensin-3-cten switch may contribute to the metastasis of mammary cancer.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms / metabolism*
  • Breast Neoplasms / pathology
  • Cell Line, Tumor
  • Cell Movement / physiology*
  • Enzyme Inhibitors / metabolism
  • Epidermal Growth Factor / metabolism*
  • ErbB Receptors
  • Female
  • Humans
  • Microfilament Proteins / genetics
  • Microfilament Proteins / metabolism*
  • Oligonucleotide Array Sequence Analysis
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / metabolism
  • Receptor, ErbB-2 / genetics
  • Receptor, ErbB-2 / metabolism
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Tensins

Substances

  • Enzyme Inhibitors
  • Microfilament Proteins
  • RNA, Small Interfering
  • Recombinant Fusion Proteins
  • TNS3 protein, human
  • TNS4 protein, human
  • Tensins
  • Epidermal Growth Factor
  • ErbB Receptors
  • Receptor, ErbB-2