Abstract
Our previous study demonstrated that successive electroacupuncture (EA) at ST36 acupoint reduces IgE production in BALB/c mice immunized with 2,4-dinitrophenylated keyhole limpet protein (DNP-KLH) by suppression of the Th2 cell lineage development. Here, we report that pretreatment of phentolamine (alpha-adrenoceptor antagonist, 10mg/kg, i.p.) completely blocks the EA-induced suppression of antigen-specific and total IgE levels in serum and IL-4 production in anti-CD3 mAb-activated splenocytes in DNP-KLH immunized mice. The results suggest that alpha-adrenoceptor play an important role in mediating the suppressive effects of EA on IgE production and Th2 cell response in DNP-KLH immunized mice.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adrenergic alpha-Antagonists / pharmacology
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Animals
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Electroacupuncture*
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Enzyme-Linked Immunosorbent Assay
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Female
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Hemocyanins / immunology
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Immunoglobulin E / biosynthesis*
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Immunoglobulin E / blood
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Interferon-gamma / biosynthesis
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Interferon-gamma / immunology
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Interleukin-4 / biosynthesis
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Interleukin-4 / immunology
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Mice
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Mice, Inbred BALB C
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Phentolamine / pharmacology
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Receptors, Adrenergic, alpha / drug effects
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Receptors, Adrenergic, alpha / immunology*
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Th2 Cells / immunology*
Substances
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Adrenergic alpha-Antagonists
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Receptors, Adrenergic, alpha
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Interleukin-4
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Immunoglobulin E
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Interferon-gamma
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Hemocyanins
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keyhole-limpet hemocyanin
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Phentolamine