Quantitative proteomics identifies surfactant-resistant alpha-synuclein in cerebral cortex of Parkinsonism-dementia complex of Guam but not Alzheimer's disease or progressive supranuclear palsy

Am J Pathol. 2007 Sep;171(3):993-1002. doi: 10.2353/ajpath.2007.070015. Epub 2007 Aug 3.

Abstract

Parkinsonism-dementia complex (PDC) remains a significant health burden to the Chamorro population. We tested the hypothesis that quantitative proteomics might provide fresh insight into this enigmatic illness by analyzing proteins resistant to surfactant extraction from patients with Alzheimer's disease (AD) or PDC and their matched controls using isobaric tags for relative and absolute quantification. In addition to the expected increase in abnormal frontal cortical Abeta peptides, tau, ubiquitin, and apolipoprotein E in AD, and tau in PDC, we identified alpha-synuclein (SNCA) as a major abnormal protein in PDC but not AD. We confirmed our isobaric tags for relative and absolute quantification findings by enzyme-linked immunosorbent assay in frontal and temporal cortices. We extended our assays to include a limited number of cases of progressive supranuclear palsy (PSP) and dementia with Lewy bodies; we observed increased abnormal tau but not SNCA in PSP, and abnormal SNCA in dementia with Lewy bodies that was quantitatively similar to PDC. Finally, soluble Abeta oligomers were selectively increased in AD but not PDC or PSP. These results show that frontal and temporal cortex in PDC is distinguished from AD and PSP by its accumulation of abnormal SNCA and suggest that PDC be considered a synucleinopathy as well as a tauopathy.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Cerebral Cortex / chemistry*
  • Cerebral Cortex / cytology
  • Guam
  • Humans
  • Middle Aged
  • Parkinsonian Disorders / pathology
  • Parkinsonian Disorders / physiopathology*
  • Proteomics*
  • Supranuclear Palsy, Progressive / pathology
  • Supranuclear Palsy, Progressive / physiopathology*
  • Surface-Active Agents / chemistry*
  • Tandem Mass Spectrometry
  • alpha-Synuclein / chemistry*

Substances

  • Surface-Active Agents
  • alpha-Synuclein