The spectrum of Trp- mutants isolated as 5-fluoroanthranilate-resistant clones in Saccharomyces bayanus, S. mikatae and S. paradoxus

Yeast. 2008 Jan;25(1):41-6. doi: 10.1002/yea.1552.

Abstract

5-Fluoroanthranilic acid (FAA)-resistant mutants were selected in homothallic diploids of three Saccharomyces species, taking care to isolate mutants of independent origin. Mutations were assigned to complementation groups by interspecific complementation with S. cerevisiae tester strains. In all three species, trp3, trp4 and trp5 mutants were recovered. trp1 mutants were also recovered if the selection was imposed on a haploid strain. Thus, FAA selection may be more generally applicable than was previously described.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aldose-Ketose Isomerases / genetics
  • Aldose-Ketose Isomerases / metabolism
  • Anthranilate Synthase / genetics
  • Anthranilate Synthase / metabolism
  • Fungal Proteins / genetics
  • Genetic Complementation Test
  • Indole-3-Glycerol-Phosphate Synthase / genetics
  • Indole-3-Glycerol-Phosphate Synthase / metabolism
  • Mutation*
  • Saccharomyces / drug effects
  • Saccharomyces / genetics*
  • Saccharomyces / isolation & purification
  • Saccharomyces / metabolism
  • Tryptophan / genetics*
  • Tryptophan / metabolism
  • ortho-Aminobenzoates / metabolism
  • ortho-Aminobenzoates / pharmacology*

Substances

  • Fungal Proteins
  • ortho-Aminobenzoates
  • Tryptophan
  • Indole-3-Glycerol-Phosphate Synthase
  • Anthranilate Synthase
  • Aldose-Ketose Isomerases