Isolation of CD24(high) and CD24(low/-) cells from MCF-7: CD24 expression is positively related with proliferation, adhesion and invasion in MCF-7

Cancer Lett. 2007 Dec 8;258(1):98-108. doi: 10.1016/j.canlet.2007.08.025.

Abstract

CD24 has been reported to have a role in metastasis of several malignancies including breast cancer. We investigated the effect of CD24 expression on biologic features in human breast cancer cells, and found that CD24 expression is associated with more rapid growth and enhanced ability of adhesion and invasion in MCF-7. The proportion of cells was higher in synthetic phase and lower in arrestic phase for CD24(high) than for CD24(low/-) cells. Cyclin D1 and p27 had stronger expressions in CD24(low/-) cells than CD24(high) cells. These suggested one possible pathway for CD24 effect on proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibiotics, Antineoplastic / pharmacology
  • Antineoplastic Agents, Hormonal / pharmacology
  • Breast Neoplasms / genetics
  • Breast Neoplasms / metabolism*
  • Breast Neoplasms / pathology
  • CD24 Antigen / genetics
  • CD24 Antigen / metabolism*
  • Cell Adhesion
  • Cell Cycle / drug effects
  • Cell Line, Tumor
  • Cell Proliferation*
  • Cyclin D
  • Cyclin-Dependent Kinase Inhibitor p27 / metabolism
  • Cyclins / metabolism
  • Doxorubicin / pharmacology
  • Estrogen Receptor alpha / metabolism
  • Female
  • Flow Cytometry
  • Humans
  • Mice
  • Neoplasm Invasiveness / pathology
  • Tamoxifen / pharmacology
  • Tumor Cells, Cultured / drug effects
  • Tumor Cells, Cultured / metabolism

Substances

  • Antibiotics, Antineoplastic
  • Antineoplastic Agents, Hormonal
  • CD24 Antigen
  • Cyclin D
  • Cyclins
  • Estrogen Receptor alpha
  • Tamoxifen
  • Cyclin-Dependent Kinase Inhibitor p27
  • Doxorubicin