ADAM9 expression is a significant and independent prognostic marker of PSA relapse in prostate cancer

Eur Urol. 2008 Nov;54(5):1097-106. doi: 10.1016/j.eururo.2007.11.034. Epub 2007 Nov 26.

Abstract

Objectives: A disintegrin and metalloprotease (ADAM) 9 has been implicated in tumour progression of prostate cancer. We evaluated the expression of ADAM9 on protein and messenger RNA (mRNA) level in a larger cohort of prostate cancer cases following prostatectomy and correlated the findings with clinicopathological parameters including prostate-specific antigen (PSA) relapse times.

Methods: We immunostained 198 clinicopathologically characterised prostate cancer cases for ADAM9. For 25 additional cases, ADAM9 mRNA of microdissected tumour and normal tissue was analysed via quantitative reverse transcriptase-polymerase chain reaction.

Results: ADAM9 was significantly upregulated in prostate cancer compared with normal tissue on mRNA and protein level. ADAM9 protein expression was significantly associated with shortened PSA relapse-free survival in univariate and multivariate analyses, particularly in patients who had received prior androgen ablation.

Conclusions: ADAM9 is overexpressed in prostate cancer cases and is an independent prognostic marker of PSA relapse-free survival following radical prostatectomy. Further studies are needed to verify its role as a predictive marker of response to androgen ablation.

Publication types

  • Comparative Study

MeSH terms

  • ADAM Proteins / biosynthesis
  • ADAM Proteins / genetics*
  • Aged
  • Biomarkers, Tumor / biosynthesis
  • Biomarkers, Tumor / blood
  • Biomarkers, Tumor / genetics
  • Blotting, Western
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Immunohistochemistry
  • Male
  • Membrane Proteins / biosynthesis
  • Membrane Proteins / genetics*
  • Middle Aged
  • Neoplasm Recurrence, Local / genetics*
  • Neoplasm Recurrence, Local / metabolism
  • Neoplasm Recurrence, Local / pathology
  • Prognosis
  • Prostate-Specific Antigen / blood*
  • Prostatectomy
  • Prostatic Neoplasms / genetics*
  • Prostatic Neoplasms / metabolism
  • Prostatic Neoplasms / surgery
  • RNA, Neoplasm / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Biomarkers, Tumor
  • Membrane Proteins
  • RNA, Neoplasm
  • Prostate-Specific Antigen
  • ADAM Proteins
  • ADAM9 protein, human