Abstract
Antibodies directed against the L1 cell adhesion protein inhibit growth of SKOV3ip human ovarian cancer cells in vitro and in vivo. Responses of SKOV3ip cells in vitro to anti-L1 mAb chCE7 and Genistein were investigated. Genistein potentiated the anti-proliferative and pro-apoptotic effects of chCE7 in SKOV3ip cells. A combination of mAb chCE7 and Genistein strongly reduced the sensitivity of p44/42 (Erk1,2) kinase, Src kinase and Akt kinase to extracellular stimulation with serum, Epidermal Growth Factor and Hepatocyte Growth Factor. The observed synergy of antibodies directed against L1 with Genistein could lead to a new therapeutic option for ovarian cancer.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal / pharmacology*
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Anticarcinogenic Agents / pharmacology*
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Apoptosis / drug effects*
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Apoptosis / immunology
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Blotting, Western
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Cell Proliferation / drug effects*
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Cell Survival / drug effects
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DNA Primers
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Drug Synergism
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Epidermal Growth Factor / pharmacology
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ErbB Receptors
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Female
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Genistein / pharmacology*
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Hepatocyte Growth Factor / pharmacology
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Humans
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Mitogen-Activated Protein Kinase 1 / metabolism
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Mitogen-Activated Protein Kinase 3 / metabolism
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Neural Cell Adhesion Molecule L1 / immunology*
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Ovarian Neoplasms / metabolism
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Ovarian Neoplasms / pathology*
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Proto-Oncogene Proteins c-akt / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Tumor Cells, Cultured
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src-Family Kinases / metabolism
Substances
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Antibodies, Monoclonal
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Anticarcinogenic Agents
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DNA Primers
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Neural Cell Adhesion Molecule L1
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RNA, Messenger
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Epidermal Growth Factor
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Hepatocyte Growth Factor
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Genistein
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ErbB Receptors
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src-Family Kinases
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Proto-Oncogene Proteins c-akt
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Mitogen-Activated Protein Kinase 1
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Mitogen-Activated Protein Kinase 3