Bioimaging of dexamethasone and TGF beta-1 and its biological activities of chondrogenic differentiation in hydrogel constructs

J Biomed Mater Res A. 2008 Nov;87(2):283-9. doi: 10.1002/jbm.a.31792.

Abstract

This study examined the efficacy of poly(NiPAAm-co-AAc) as an injectable drug delivery vehicle and a cell therapeutic agent in the form of a supporting matrix for the chondrogenic differentiation of rabbit chondrocytes. The hydrogel constructs, which consisted of embedded cells co-encapsulating dexamethasone (Dex) and TGF beta-1 or unloaded Dex, were used as controls to determine the effects of Dex and TGF beta-1 on chondrogenic differentiation. The level of Dex and TGF beta-1 released was monitored using a bioimaging method. The amount of Dex released from hydrogel was faster than that of TGF beta-1. TGF beta-1 was present in hydrogel for more than 4 weeks after the injection. The level of the cartilage associated ECM proteins was examined by immunohistochemical staining for collagen type II as well as by Safranin-O and Alcian blue (GAG) staining. These results highlight the potential of a thermo-reversible hydrogel mixed with the chondrocytes and differentiation delivery material for applications in neocartilage formation.

Publication types

  • Evaluation Study

MeSH terms

  • Acrylic Resins / chemistry
  • Acrylic Resins / metabolism
  • Animals
  • Biocompatible Materials / chemistry
  • Biocompatible Materials / metabolism
  • Carbocyanines / chemistry
  • Carbocyanines / metabolism
  • Cell Differentiation / drug effects*
  • Cells, Cultured
  • Chondrocytes / cytology
  • Chondrocytes / drug effects*
  • Chondrocytes / physiology*
  • Chondrogenesis / drug effects*
  • Collagen Type II / genetics
  • Collagen Type II / metabolism
  • Dexamethasone / chemistry
  • Dexamethasone / metabolism
  • Dexamethasone / pharmacology*
  • Drug Carriers / chemistry
  • Drug Carriers / metabolism
  • Female
  • Fluorescein-5-isothiocyanate / chemistry
  • Fluorescein-5-isothiocyanate / metabolism
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / metabolism
  • Glucocorticoids / chemistry
  • Glucocorticoids / metabolism
  • Glucocorticoids / pharmacology
  • Humans
  • Hydrogels / chemistry*
  • Male
  • Materials Testing
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Rabbits
  • Tissue Engineering / methods
  • Transforming Growth Factor beta1 / chemistry
  • Transforming Growth Factor beta1 / metabolism
  • Transforming Growth Factor beta1 / pharmacology*

Substances

  • Acrylic Resins
  • Biocompatible Materials
  • CY5.5 cyanine dye
  • Carbocyanines
  • Collagen Type II
  • Drug Carriers
  • Fluorescent Dyes
  • Glucocorticoids
  • Hydrogels
  • Transforming Growth Factor beta1
  • poly(N-isopropylacrylamide-co-acrylamide)
  • Dexamethasone
  • Fluorescein-5-isothiocyanate