The septins function in G1 pathways that influence the pattern of cell growth in budding yeast

PLoS One. 2008 Apr 23;3(4):e2022. doi: 10.1371/journal.pone.0002022.

Abstract

The septins are a conserved family of proteins that have been proposed to carry out diverse functions. In budding yeast, the septins become localized to the site of bud emergence in G1 but have not been thought to carry out important functions at this stage of the cell cycle. We show here that the septins function in redundant mechanisms that are required for formation of the bud neck and for the normal pattern of cell growth early in the cell cycle. The Shs1 septin shows strong genetic interactions with G1 cyclins and is directly phosphorylated by G1 cyclin-dependent kinases, consistent with a role in early cell cycle events. However, Shs1 phosphorylation site mutants do not show genetic interactions with the G1 cyclins or obvious defects early in the cell cycle. Rather, they cause an increased cell size and aberrant cell morphology that are dependent upon inhibitory phosphorylation of Cdk1 at the G2/M transition. Shs1 phosphorylation mutants also show defects in interaction with the Gin4 kinase, which associates with the septins during G2/M and plays a role in regulating inhibitory phosphorylation of Cdk1. Phosphorylation of Shs1 by G1 cyclin-dependent kinases plays a role in events that influence Cdk1 inhibitory phosphorylation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cell Proliferation
  • Consensus Sequence
  • Cyclin-Dependent Kinases / metabolism
  • Cyclins / metabolism
  • G1 Phase*
  • Molecular Sequence Data
  • Mutation / genetics
  • Peptide Mapping
  • Phosphorylation
  • Protein Binding
  • Protein Transport
  • Saccharomyces cerevisiae Proteins / chemistry
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Saccharomycetales / cytology*
  • Saccharomycetales / enzymology

Substances

  • CLN1 protein, S cerevisiae
  • CLN2 protein, S cerevisiae
  • Cyclins
  • Saccharomyces cerevisiae Proteins
  • Cyclin-Dependent Kinases