Expression of somatostatin and somatostatin receptor subtypes 1-5 in human normal and diseased kidney

J Histochem Cytochem. 2008 Aug;56(8):733-43. doi: 10.1369/jhc.2008.950998. Epub 2008 Apr 28.

Abstract

Somatostatin mediates inhibitory functions through five G protein-coupled somatostatin receptors (sst1-5). We used immunohistochemistry, immunofluorescence, and RT-PCR to determine the presence of somatostatin receptors sst1, sst2A, sst2B, sst3, sst4, and sst5 in normal and IgA nephropathy human kidney. All somatostatin receptors were detected in the thin tubules (distal convoluted tubules and loops of Henle) and thick tubules (proximal convoluted tubules) in the tissue sections from nephrectomy and biopsy samples. Immunopositive sst1 and sst4 staining was more condensed in the cytoplasm of tubular epithelial cells. In normal kidney tissue sections, podocytes and mesangial cells in the glomeruli stained for sst1, sst2B, sst4 and sst5, and stained weakly for sst3. In IgA kidney tissue, the expression of somatostatin receptors was significantly increased with particular immmunopositive staining for sst1, sst2B, sst4, and sst5 within glomeruli. In the epithelial cells, the staining for sst2B and sst4 in proximal tubules and sst1, sst2B, and sst5 in distal tubules was increased. The mRNA expression of sst1-5 was also detected by RT-PCR. Somatostatin and all five receptor subtypes were ubiquitously distributed in normal kidney and IgA nephropathy. The increased expression of somatostatin receptors in IgA nephropathy kidney might be the potential pathogenesis of inflammatory renal disease.

MeSH terms

  • Glomerulonephritis, IGA / metabolism*
  • Humans
  • Immunohistochemistry
  • Kidney / metabolism*
  • RNA, Messenger / biosynthesis
  • Receptors, Somatostatin / biosynthesis*
  • Receptors, Somatostatin / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Somatostatin / biosynthesis*

Substances

  • RNA, Messenger
  • Receptors, Somatostatin
  • somatostatin receptor 3
  • somatostatin receptor subtype-4
  • somatostatin receptor type 1
  • Somatostatin
  • somatostatin receptor 5
  • somatostatin receptor 2