Abstract
Defined segments of the transmembrane envelope glycoprotein (gp45) of equine infectious anemia virus were expressed as TrpLE fusion proteins and examined for their reactivity in Western immunoblots against a diverse panel of equine immune sera. The most immunogenic region of gp45 was localized to its amino terminus, positioned between the hydrophobic fusion and the transmembrane domains. A series of overlapping synthetic peptides were used in enzyme-linked immunosorbent assays to define an immunodominant epitope within this region. In contrast, the carboxy-terminal half of gp45 displayed both weak and variable immunoreactivity with equine immune sera.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antibody Formation*
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Cell Membrane / ultrastructure
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DNA, Viral / genetics
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Equine Infectious Anemia / immunology
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Genes, Viral
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Horses / immunology*
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Immune Sera / immunology
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Infectious Anemia Virus, Equine / genetics
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Infectious Anemia Virus, Equine / immunology*
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Models, Structural
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Molecular Sequence Data
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Protein Conformation
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Recombinant Fusion Proteins / immunology
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Restriction Mapping
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / immunology*
Substances
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DNA, Viral
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Immune Sera
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Recombinant Fusion Proteins
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Viral Envelope Proteins
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transmembrane envelope glycoprotein gp45, retrovirus