Rapid identification of penicillin and macrolide resistance genes and simultaneous quantification of Streptococcus pneumoniae in purulent sputum samples by use of a novel real-time multiplex PCR assay

Kazuko Y Fukushima; Katsunori Yanagihara; Yoichi Hirakata; Kazuyuki Sugahara; Yoshitomo Morinaga; Shigeru Kohno; Shimeru Kamihira

doi:10.1128/JCM.00051-08

Rapid identification of penicillin and macrolide resistance genes and simultaneous quantification of Streptococcus pneumoniae in purulent sputum samples by use of a novel real-time multiplex PCR assay

J Clin Microbiol. 2008 Jul;46(7):2384-8. doi: 10.1128/JCM.00051-08. Epub 2008 May 7.

Authors

Kazuko Y Fukushima¹, Katsunori Yanagihara, Yoichi Hirakata, Kazuyuki Sugahara, Yoshitomo Morinaga, Shigeru Kohno, Shimeru Kamihira

Affiliation

¹ Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki City 852-8501, Japan.

Abstract

We evaluated a real-time quantitative PCR combined with a multiplex PCR assay for the quantification of Streptococcus pneumoniae and the simultaneous detection of drug-resistant genes by gel-based PCR, using purulent sputum samples. This assay correctly quantified S. pneumoniae and identified their penicillin and erythromycin susceptibilities directly from samples within 3 h.

Publication types

Evaluation Study

MeSH terms

Anti-Bacterial Agents / pharmacology
Colony Count, Microbial / methods*
DNA, Bacterial / genetics*
Drug Resistance, Bacterial / genetics*
Electrophoresis, Agar Gel
Humans
Macrolides / pharmacology
Microbial Sensitivity Tests
Penicillins / pharmacology
Pneumococcal Infections / microbiology
Polymerase Chain Reaction / methods*
Sputum / microbiology*
Streptococcus pneumoniae / drug effects*
Streptococcus pneumoniae / genetics
Streptococcus pneumoniae / isolation & purification*
Suppuration / microbiology

Substances

Anti-Bacterial Agents
DNA, Bacterial
Macrolides
Penicillins