Abstract
Inactivation of Artemia salina and rabbit ribosomes by gelonin requires ATP and a high-Mr factor present in the rabbit reticulocyte-lysate post-ribosomal supernatant. The kinetic constants of the gelonin-catalysed release of adenine from A. salina ribosomes are Km = 4.35 microM and Kcat. = 0.1 min-1 in the absence of cofactors, and Km = 1.15 microM and Kcat. = 108 min-1 in their presence. The last two values are similar to those measured for ricin A chain in the absence of cofactors (Km = 2.02 microM and Kcat. = 317 min-1).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenine / metabolism
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Animals
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Artemia
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Kinetics
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Plant Proteins / pharmacology*
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Poly U / metabolism
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Protein Biosynthesis / drug effects
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Protein Synthesis Inhibitors / pharmacology*
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RNA, Messenger / genetics
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Rabbits
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Ribosome Inactivating Proteins, Type 1
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Ribosomes / drug effects*
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Ribosomes / metabolism
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Ricin / pharmacology
Substances
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Plant Proteins
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Protein Synthesis Inhibitors
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RNA, Messenger
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Ribosome Inactivating Proteins, Type 1
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Poly U
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GEL protein, Gelonium multiflorum
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Ricin
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Adenine