Viral double-stranded RNA triggers Ig class switching by activating upper respiratory mucosa B cells through an innate TLR3 pathway involving BAFF

J Immunol. 2008 Jul 1;181(1):276-87. doi: 10.4049/jimmunol.181.1.276.

Abstract

Class switch DNA recombination (CSR) from IgM to IgG and IgA is crucial for antiviral immunity. Follicular B cells undergo CSR upon engagement of CD40 by CD40 ligand on CD4+ T cells. This T cell-dependent pathway requires 5-7 days, which is too much of a delay to block quickly replicating pathogens. To compensate for this limitation, extrafollicular B cells rapidly undergo CSR through a T cell-independent pathway that involves innate Ag receptors of the TLR family. We found that a subset of upper respiratory mucosa B cells expressed TLR3 and responded to viral dsRNA, a cognate TLR3 ligand. In the presence of dsRNA, mucosal B cells activated NF-kappaB, a transcription factor critical for CSR. Activation of NF-kappaB required TRIF (Toll/IL-1R domain-containing protein inducing IFN-beta), a canonical TLR3 adapter protein, and caused germline transcription of downstream CH genes as well as expression of AID (activation-induced cytidine deaminase), a DNA-editing enzyme essential for CSR. Subsequent IgG and IgA production was enhanced by BAFF (B cell-activating factor of the TNF family), an innate mediator released by TLR3-expressing mucosal dendritic cells. Indeed, these innate immune cells triggered IgG and IgA responses upon exposure to dsRNA. By showing active TLR3 signaling and ongoing CSR in upper respiratory mucosa B cells from patients with CD40 signaling defects, our findings indicate that viral dsRNA may initiate frontline IgG and IgA responses through an innate TLR3-dependent pathway involving BAFF.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Vesicular Transport / metabolism
  • B-Cell Activating Factor / metabolism*
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology*
  • B-Lymphocytes / metabolism
  • CD40 Antigens / immunology
  • Cell Proliferation
  • Cells, Cultured
  • Cytidine Deaminase / metabolism
  • Dendritic Cells / immunology
  • Dendritic Cells / metabolism
  • Humans
  • Immunoglobulin Class Switching / genetics
  • Immunoglobulin Class Switching / immunology*
  • Immunoglobulins / genetics
  • Immunoglobulins / immunology*
  • Immunoglobulins / metabolism
  • Mucous Membrane / cytology
  • Mucous Membrane / immunology
  • RNA, Double-Stranded / genetics
  • Signal Transduction / immunology*
  • Toll-Like Receptor 3 / metabolism*
  • Up-Regulation

Substances

  • Adaptor Proteins, Vesicular Transport
  • B-Cell Activating Factor
  • CD40 Antigens
  • Immunoglobulins
  • RNA, Double-Stranded
  • TICAM1 protein, human
  • TLR3 protein, human
  • Toll-Like Receptor 3
  • AICDA (activation-induced cytidine deaminase)
  • Cytidine Deaminase