Abstract
Objective:
To detect TB specific T cell responses by using the recombinant ESAT-6 protein as stimulus in Chinese HIV infected patients.
Methods:
ELISPOT-IFN-gamma assay by using the recombinant ESAT-6 protein as stimulus to detect specific T cell responses in HIV+ patients with or without clinical manifestation of TB diseases.
Results:
Recombinant ESAT-6 protein specific T cell responses show significant high frequencies in both of TB patients with or without HIV infection than that in the healthy control and HIV+ group without clinical TB diseases.
Conclusion:
The ELISPOT-IFN-gamma assay by using recombinant ESAT-6 protein as stimulus could be used in diagnoses of TB infection in Chinese HIV infected patients.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Aged, 80 and over
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Antigens, Bacterial / genetics
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Antigens, Bacterial / immunology*
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Bacterial Proteins / genetics
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Bacterial Proteins / immunology*
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Female
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HIV Infections / blood
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HIV Infections / immunology*
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Humans
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Immunoenzyme Techniques / methods
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Interferon-gamma / metabolism
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Leukocytes, Mononuclear / cytology
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Leukocytes, Mononuclear / metabolism
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Male
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Middle Aged
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Recombinant Proteins / immunology
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T-Lymphocytes / cytology
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T-Lymphocytes / metabolism
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Tuberculosis / blood
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Tuberculosis / immunology*
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Young Adult
Substances
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Antigens, Bacterial
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Bacterial Proteins
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ESAT-6 protein, Mycobacterium tuberculosis
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Recombinant Proteins
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Interferon-gamma