Capabilities of microbore columns coupled to inductively coupled plasma mass spectrometry in speciation of arsenic and selenium

J Chromatogr A. 2008 Aug 22;1202(2):132-7. doi: 10.1016/j.chroma.2008.06.031. Epub 2008 Jul 1.

Abstract

The performance of microHPLC-microconcentric nebulizer-inductively coupled plasma-mass spectrometry (ICP-MS) coupling for the simultaneous determination of As(III), As(V), monomethylarsenic acid (MMA), dimethylarsinic acid (DMA), selenite (SeIV) and selenate (SeVI) in water was evaluated. The accurate reduction of the off-column dead volume, specially the capillary of the micronebulizer, as well as the optimization of chromatographic conditions led to the claimed advantages expected for microbore columns: a significant diminution of sample and solvent consumption without sacrificing sensitivity and the overall resolution in faster analysis time (less than 5 min). Detection limits are in the range 0.03-0.04 microg L(-1) for arsenic species and 0.35 microg L(-1) for selenium species. The developed method was validated by analysing different spiked environmental water samples. Linearity, tested up to 50 microg L(-1), showed correlation coefficients above 0.999 and no matrix effect for high saline water samples. Good accuracy and repeatability was obtained for spiked influent and effluent water treatment plant.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arsenic / analysis*
  • Cacodylic Acid / analysis
  • Chromatography, High Pressure Liquid / instrumentation
  • Chromatography, High Pressure Liquid / methods*
  • Mass Spectrometry / methods*
  • Reproducibility of Results
  • Selenic Acid
  • Selenium / analysis*
  • Selenium Compounds / analysis
  • Sodium Selenite / analysis
  • Water Pollutants, Chemical / analysis
  • Water Supply / analysis

Substances

  • Selenium Compounds
  • Water Pollutants, Chemical
  • Cacodylic Acid
  • Selenium
  • Sodium Selenite
  • Selenic Acid
  • Arsenic