Abstract
Glyburide and tolbutamide, at concentrations of 20 to 40 mumol/L and 1 to 2 mmol/L, respectively, stimulated glucose transport in rat adipocytes. Concomitantly, protein kinase C was activated, as evidenced by translocation of immunoreactive enzyme from cytosol to membranes. Glucose transport effects of the sulfonylureas were blocked by three inhibitors of protein kinase C (H-7, staurosporine, and sangivamycin), and by phorbol ester-induced down-regulation of protein kinase C. These findings suggest that sulfonylureas may stimulate glucose transport in rat adipocytes through activation of protein kinase C.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adipose Tissue / cytology
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Adipose Tissue / enzymology*
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Animals
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Biological Transport / drug effects
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Deoxyglucose / metabolism
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Glucose / metabolism*
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Glyburide / pharmacology
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Insulin / pharmacology
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Male
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Protein Kinase C / antagonists & inhibitors
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Protein Kinase C / metabolism*
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Rats
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Rats, Inbred Strains
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Sulfonylurea Compounds / pharmacology*
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Tetradecanoylphorbol Acetate / pharmacology
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Time Factors
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Tolbutamide / pharmacology
Substances
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Insulin
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Sulfonylurea Compounds
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Tolbutamide
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Deoxyglucose
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Protein Kinase C
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Glucose
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Tetradecanoylphorbol Acetate
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Glyburide