Abstract
We describe a method based on a phagemid vector with helper phage rescue for the construction and rapid analysis of combinatorial antibody Fab libraries. This approach should allow the generation and selection of many monoclonal antibodies. Antibody genes are expressed in concert with phage morphogenesis, thereby allowing incorporation of functional Fab molecules along the surface of filamentous phage. The power of the method depends upon the linkage of recognition and replication functions and is not limited to antibody molecules.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antibodies, Monoclonal / genetics
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Bacteriophages / genetics*
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Bacteriophages / immunology
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Bacteriophages / physiology
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Enzyme-Linked Immunosorbent Assay
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Genetic Vectors
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Immunoglobulin Fab Fragments / genetics*
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Immunoglobulin Heavy Chains / genetics
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Immunoglobulin kappa-Chains / genetics
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Immunosorbent Techniques
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Microscopy, Electron
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Tetanus / immunology
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Virus Replication
Substances
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Antibodies, Monoclonal
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Immunoglobulin Fab Fragments
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Immunoglobulin Heavy Chains
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Immunoglobulin kappa-Chains