Abstract
Cytoplasmic male sterility (CMS) has often been associated with abnormal mitochondrial open reading frames (ORF), orfH79 is a mitochondria chimeric gene being responsible for the CMS trait in Honglian (HL) rice. Weakly expressed ORFH79 strongly inhibits the growth of yeast cells. In addition, the content of reactive oxygen species (ROS) in the transformants that expressed ORFH79 was increased by 31%, and ATP was decreased by 41% compared with the control. These results showed ORFH79 peptide is toxic to yeast cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphate / metabolism*
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Cloning, Molecular
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Gene Expression
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Mitochondrial Proteins / genetics
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Mitochondrial Proteins / toxicity*
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Oryza / genetics*
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Plant Proteins / genetics
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Plant Proteins / toxicity*
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Reactive Oxygen Species / metabolism*
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Recombinant Proteins / genetics
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Recombinant Proteins / toxicity
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / growth & development*
Substances
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Mitochondrial Proteins
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Plant Proteins
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Reactive Oxygen Species
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Recombinant Proteins
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Adenosine Triphosphate