Immunohistochemical and proteomic profile of melanotic medulloblastoma

Pediatr Blood Cancer. 2009 Jul;52(7):875-7. doi: 10.1002/pbc.21887.

Abstract

We present the case of a 6-year-old male affected by an infratentorial tumor. Histological diagnosis was melanotic medulloblastoma. Immunohistochemistry showed in the melanin rich areas positive cells for HMB45. We performed a proteomic study to compare protein profiles in melanotic versus non-melanotic areas. Protein profiles of different areas of the tumor displayed similarity, with the exception of seven proteins. In accordance with the hypothesis that melanotic medulloblastomas produce oculo-cutaneous melanin, proteomic analysis showed melanocytic-associated antigens and epidermal autoantigen 450K in the pigmented nodule; both these proteins have a significant role as markers of melanotic elements.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cerebellar Neoplasms / metabolism*
  • Cerebellar Neoplasms / pathology
  • Child
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Immunoenzyme Techniques
  • Male
  • Mass Spectrometry
  • Medulloblastoma / metabolism*
  • Medulloblastoma / pathology
  • Melanins / metabolism*
  • Neoplasm Proteins / metabolism*
  • Proteomics*

Substances

  • Melanins
  • Neoplasm Proteins