An inherited mitochondrial DNA disruptive mutation shifts to homoplasmy in oncocytic tumor cells

Hum Mutat. 2009 Mar;30(3):391-6. doi: 10.1002/humu.20870.

Abstract

A disruptive frameshift mtDNA mutation affecting the ND5 subunit of complex I is present in homoplasmy in a nasopharyngeal oncocytic tumor and inherited as a heteroplasmic germline mutation recurring in two of the patient's siblings. Homoplasmic ND5 mutation in the tumor correlates with lack of the ND6 subunit, suggesting complex I disassembly. A few oncocytic areas, expressing ND6 and heteroplasmic for the ND5 mutation, harbor a de novo homoplasmic ND1 mutation. Since shift to homoplasmy of ND1 and ND5 mutations occurs exclusively in tumor cells, we conclude that complex I mutations may have a selective advantage and accompany oncocytic transformation.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Western
  • DNA Mutational Analysis
  • DNA, Mitochondrial / chemistry
  • DNA, Mitochondrial / genetics*
  • Electron Transport Complex I / genetics*
  • Electron Transport Complex I / metabolism
  • Frameshift Mutation*
  • Germ-Line Mutation
  • Humans
  • Immunohistochemistry
  • Male
  • Microscopy, Electron
  • Molecular Sequence Data
  • Nasopharyngeal Neoplasms / genetics*
  • Nasopharyngeal Neoplasms / pathology
  • Nasopharyngeal Neoplasms / ultrastructure
  • Pedigree
  • Sequence Deletion
  • Sequence Homology, Amino Acid
  • Siblings

Substances

  • DNA, Mitochondrial
  • Electron Transport Complex I