Abstract
We established a real-time PCR assay with melting curve analysis to rapidly genotype quinolone resistance-determining regions (QRDRs) of gyrase A and topoisomerase IV genes in Haemophilus influenzae. This assay is a useful tool for the detection of fluoroquinolone resistance and for the early detection of preexisting QRDR mutations.
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / genetics
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DNA Gyrase / genetics
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DNA Topoisomerase IV / genetics*
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DNA, Bacterial / genetics*
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Drug Resistance, Bacterial
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Haemophilus influenzae / enzymology*
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Haemophilus influenzae / genetics*
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Humans
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Microbial Sensitivity Tests / methods
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Mutation*
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Polymerase Chain Reaction / methods
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Quinolones / pharmacology
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Transition Temperature*
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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DNA, Bacterial
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Quinolones
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DNA Topoisomerase IV
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DNA Gyrase