Sperm chromosome analysis of an infertile patient with a 95% mosaic r(21) karyotype and normal phenotype

Fertil Steril. 2009 Mar;91(3):930.e13-5. doi: 10.1016/j.fertnstert.2008.12.005. Epub 2009 Jan 10.

Abstract

Objective: To examine sperm meiotic segregation in a man with mosaic ring chromosome 21.

Design: Case report.

Setting: Hospital departments of reproductive biology, cytogenetics, gynecology, and obstetrics.

Patient(s): One patient referred for cryptozoospermia, heterozygous for a ring chromosome.

Intervention(s): Fluorescence in situ hybridization with chromosome 21-specific probes after sperm selection.

Result(s): A total of 169 spermatozoa were selected; 92.3% carried a normal 21 chromosome, 6.5% the ring chromosome, and 1.2% both.

Conclusion(s): Ring chromosome frequency in mature sperm cells was low and may be due to preferential meiosis of normal spermatogonia,which could explain the cryptozoospermia and unexpected ratio in this case.

Publication types

  • Case Reports

MeSH terms

  • Chromosome Segregation
  • Chromosomes, Human, Pair 21*
  • Down Syndrome / genetics
  • Genotype
  • Humans
  • In Situ Hybridization, Fluorescence*
  • Infertility, Male / genetics*
  • Infertility, Male / physiopathology
  • Karyotyping*
  • Male
  • Meiosis / genetics
  • Mosaicism*
  • Phenotype
  • Reproductive Techniques, Assisted
  • Ring Chromosomes*
  • Risk Assessment
  • Semen Analysis*
  • Sperm Count
  • Sperm Motility
  • Spermatogenesis / genetics*