Zinc activates damage-sensing TRPA1 ion channels

Nat Chem Biol. 2009 Mar;5(3):183-90. doi: 10.1038/nchembio.146. Epub 2009 Feb 8.

Abstract

Zinc is an essential biological trace element. It is required for the structure or function of over 300 proteins, and it is increasingly recognized for its role in cell signaling. However, high concentrations of zinc have cytotoxic effects, and overexposure to zinc can cause pain and inflammation through unknown mechanisms. Here we show that zinc excites nociceptive somatosensory neurons and causes nociception in mice through TRPA1, a cation channel previously shown to mediate the pungency of wasabi and cinnamon through cysteine modification. Zinc activates TRPA1 through a unique mechanism that requires zinc influx through TRPA1 channels and subsequent activation via specific intracellular cysteine and histidine residues. TRPA1 is highly sensitive to intracellular zinc, as low nanomolar concentrations activate TRPA1 and modulate its sensitivity. These findings identify TRPA1 as an important target for the sensory effects of zinc and support an emerging role for zinc as a signaling molecule that can modulate sensory transmission.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Humans
  • Mice
  • Mice, Knockout
  • Mutagenesis, Site-Directed
  • Pain / chemically induced
  • Protein Conformation
  • Sensory Receptor Cells / drug effects
  • Sensory Receptor Cells / metabolism
  • Sensory Receptor Cells / physiology
  • TRPA1 Cation Channel
  • Transient Receptor Potential Channels / chemistry
  • Transient Receptor Potential Channels / drug effects*
  • Transient Receptor Potential Channels / genetics
  • Transient Receptor Potential Channels / physiology
  • Zinc / pharmacokinetics
  • Zinc / toxicity*

Substances

  • TRPA1 Cation Channel
  • Transient Receptor Potential Channels
  • Trpa1 protein, mouse
  • Zinc

Associated data

  • PubChem-Substance/56458795
  • PubChem-Substance/56458796
  • PubChem-Substance/56458797
  • PubChem-Substance/56458798
  • PubChem-Substance/56458799
  • PubChem-Substance/56458800
  • PubChem-Substance/56458801
  • PubChem-Substance/56458802
  • PubChem-Substance/56458803
  • PubChem-Substance/56458804
  • PubChem-Substance/56458805
  • PubChem-Substance/56458806