The structure of NMB1585, a MarR-family regulator from Neisseria meningitidis

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Mar 1;65(Pt 3):204-9. doi: 10.1107/S174430910900414X. Epub 2009 Feb 26.

Abstract

The structure of the MarR-family transcription factor NMB1585 from Neisseria meningitidis has been solved using data extending to a resolution of 2.1 A. Overall, the dimeric structure resembles those of other MarR proteins, with each subunit comprising a winged helix-turn-helix (wHtH) domain connected to an alpha-helical dimerization domain. The spacing of the recognition helices of the wHtH domain indicates that NMB1585 is pre-configured for DNA binding, with a putative inducer pocket that is largely occluded by the side chains of two aromatic residues (Tyr29 and Trp53). NMB1585 was shown to bind to its own promoter region in a gel-shift assay, indicating that the protein acts as an auto-repressor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / chemistry*
  • Binding Sites
  • Crystallography, X-Ray
  • DNA, Bacterial / metabolism
  • Escherichia coli / chemistry
  • Models, Molecular
  • Molecular Sequence Data
  • Neisseria meningitidis / chemistry*
  • Protein Binding
  • Sequence Alignment

Substances

  • Bacterial Proteins
  • DNA, Bacterial