Antibody recognition force microscopy shows that outer membrane cytochromes OmcA and MtrC are expressed on the exterior surface of Shewanella oneidensis MR-1

Appl Environ Microbiol. 2009 May;75(9):2931-5. doi: 10.1128/AEM.02108-08. Epub 2009 Mar 13.

Abstract

Antibody recognition force microscopy showed that OmcA and MtrC are expressed on the exterior surface of living Shewanella oneidensis MR-1 cells when Fe(III), including solid-phase hematite (Fe(2)O(3)), was the terminal electron acceptor. OmcA was localized to the interface between the cell and mineral. MtrC displayed a more uniform distribution across the cell surface. Both cytochromes were associated with an extracellular polymeric substance.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antibodies / metabolism
  • Bacterial Proteins / analysis*
  • Cytochrome c Group / analysis*
  • Cytochromes / analysis*
  • Ferric Compounds / metabolism
  • Membrane Proteins / analysis*
  • Microscopy, Atomic Force
  • Shewanella / chemistry*

Substances

  • Antibodies
  • Bacterial Proteins
  • Cytochrome c Group
  • Cytochromes
  • Ferric Compounds
  • Membrane Proteins
  • ferric oxide
  • MtrC protein, Shewanella