Molecules that promote or enhance reprogramming of somatic cells to induced pluripotent stem cells

Cell Stem Cell. 2009 Apr 3;4(4):301-12. doi: 10.1016/j.stem.2009.03.005.

Abstract

Reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) can be achieved by viral-mediated transduction of defined transcription factors. Moving toward the eventual goal of clinical application, it is necessary to overcome limitations such as low reprogramming efficiency and genomic alterations due to viral integration. Here, we review recent progress made in the usage of genetic factors, chemical inhibitors, and signaling molecules that can either replace core reprogramming factors or enhance reprogramming efficiency. Current iPSC studies will provide a paradigm for the combinatorial use of genetic factors and chemicals for the broader applications to alter cellular states of potency.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Cell Differentiation / drug effects*
  • Cell Differentiation / physiology
  • Cellular Reprogramming / drug effects*
  • Cellular Reprogramming / genetics*
  • Cellular Reprogramming / physiology
  • DNA (Cytosine-5-)-Methyltransferases / antagonists & inhibitors
  • DNA (Cytosine-5-)-Methyltransferases / metabolism
  • Enzyme Inhibitors / pharmacology
  • Glycogen Synthase Kinase 3 / antagonists & inhibitors
  • Glycogen Synthase Kinase 3 / metabolism
  • Histone Deacetylase Inhibitors
  • Histone Deacetylases / metabolism
  • Homeodomain Proteins / antagonists & inhibitors
  • Homeodomain Proteins / metabolism
  • Humans
  • MAP Kinase Kinase Kinases / antagonists & inhibitors
  • MAP Kinase Kinase Kinases / metabolism
  • MicroRNAs / pharmacology
  • Octamer Transcription Factor-3 / antagonists & inhibitors
  • Octamer Transcription Factor-3 / metabolism
  • Pluripotent Stem Cells / drug effects
  • Pluripotent Stem Cells / physiology*
  • Signal Transduction / drug effects*
  • Signal Transduction / physiology
  • Transforming Growth Factor beta / antagonists & inhibitors
  • Transforming Growth Factor beta / metabolism
  • Wnt Proteins / agonists
  • Wnt Proteins / metabolism

Substances

  • Enzyme Inhibitors
  • Histone Deacetylase Inhibitors
  • Homeodomain Proteins
  • MicroRNAs
  • Octamer Transcription Factor-3
  • Transforming Growth Factor beta
  • Wnt Proteins
  • DNA (Cytosine-5-)-Methyltransferases
  • MAP Kinase Kinase Kinases
  • Glycogen Synthase Kinase 3
  • Histone Deacetylases