Resveratrol attenuates angiotensin II-induced interleukin-6 expression and perivascular fibrosis

Hypertens Res. 2009 Jun;32(6):466-71. doi: 10.1038/hr.2009.47. Epub 2009 Apr 17.

Abstract

Recent studies have shown that resveratrol (3,5,4'-trihydroxystilbene), a polyphenolic compound found in grapes and red wine, has various beneficial effects on cardiovascular diseases and prolongs the life span of mice fed a high-fat diet. We hypothesized that resveratrol may attenuate vascular inflammatory response induced by angiotensin (Ang) II. We examined the effect of resveratrol on Ang II-induced interleukin (IL)-6 expression in vascular smooth muscle cells (VSMCs). Resveratrol significantly attenuated Ang II-induced IL-6 mRNA expression and IL-6 protein in the supernatant of VSMC in a dose-dependent manner. Resveratrol suppressed the IL-6 gene promoter activity. Resveratrol inhibited the Ang II-induced cAMP-response element-binding protein and nuclear factor-kappa B activity, which are critical for Ang II-induced IL-6 gene activation. An increase in the serum concentration of IL-6 induced by Ang II infusion was attenuated by an oral administration of resveratrol. Resveratrol also inhibited Ang II-induced hypertension and perivascular fibrosis of the heart. Although hydralazine reduced blood pressure level equal to resveratrol, it did not reduce the Ang II-induced IL-6 production and perivascular fibrosis. These data suggest that the inhibition of Ang II-induced vascular inflammation and high blood pressure by resveratrol may contribute, at least in part, to the anti-atherogenic effects of resveratrol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiotensin II / antagonists & inhibitors*
  • Angiotensin II / toxicity
  • Animals
  • Antioxidants / pharmacology*
  • Blood Pressure / drug effects
  • Blotting, Northern
  • Blotting, Western
  • Body Weight / drug effects
  • Cells, Cultured
  • DNA / biosynthesis
  • DNA / genetics
  • Electrophoretic Mobility Shift Assay
  • Enzyme-Linked Immunosorbent Assay
  • Fibrosis
  • Heart Rate / drug effects
  • Interleukin-6 / biosynthesis*
  • Luciferases / genetics
  • Mice
  • Mice, Inbred C57BL
  • Muscle, Smooth, Vascular / cytology
  • Muscle, Smooth, Vascular / drug effects
  • Promoter Regions, Genetic / genetics
  • Rats
  • Resveratrol
  • Stilbenes / pharmacology*
  • Vascular Diseases / chemically induced
  • Vascular Diseases / pathology
  • Vascular Diseases / prevention & control*

Substances

  • Antioxidants
  • Interleukin-6
  • Stilbenes
  • Angiotensin II
  • DNA
  • Luciferases
  • Resveratrol