Interstitial pregnancy is a rare form of ectopic pregnancy, but it results in significant maternal morbidity and mortality. In this paper, describe a case of interstitial pregnancy that was successfully managed by laparoscopic occlusion of the ipsilateral ascending branch of uterine artery and the communication of the ovarian artery. It would help to minimize the active bleeding and allow removing the gestation sac, placental tissue, and repair the interstitial tube. In this case, the operative bleeding was 80 mL only, and the operative time was 70 minutes. The patient shared good postoperative recovery, and serum beta human chorionic gonadotropin decreased to below normal levels within 1 month after the operation. This procedure should be considered as a treatment option for unruptured interstitial pregnancy and may allow for conserving the patient's uterus and fallopian tube and preserving the future fertility.