A xenon-129 biosensor for monitoring MHC-peptide interactions

Angew Chem Int Ed Engl. 2009;48(23):4142-5. doi: 10.1002/anie.200806149.

Abstract

Caged in: The formation of a complex between a peptide ligand and a major histocompatibility complex (MHC) class II protein is detected by a (129)Xe biosensor. Cryptophane molecules that trap Xe atoms are modified with a hemagglutinin (HA) peptide, which binds to the MHC protein. The interaction can be monitored by an NMR chemical shift change of cage-HA bound (129)Xe.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques*
  • Cell Line
  • Hemagglutinins / chemistry
  • Histocompatibility Antigens Class II / chemistry
  • Histocompatibility Antigens Class II / metabolism*
  • Humans
  • Macromolecular Substances / chemistry
  • Nuclear Magnetic Resonance, Biomolecular
  • Polycyclic Compounds
  • Protein Interaction Mapping / methods*
  • Triazoles / chemistry
  • Xenon Isotopes / chemistry*

Substances

  • Hemagglutinins
  • Histocompatibility Antigens Class II
  • Macromolecular Substances
  • Polycyclic Compounds
  • Triazoles
  • Xenon Isotopes
  • cryptophane