Use of a new single multiplex PCR-based assay for direct simultaneous characterization of six Neisseria meningitidis serogroups

Christophe Fraisier; Richard Stor; Bernard Tenebray; Yannick Sanson; Pierre Nicolas

doi:10.1128/JCM.02415-08

Use of a new single multiplex PCR-based assay for direct simultaneous characterization of six Neisseria meningitidis serogroups

J Clin Microbiol. 2009 Aug;47(8):2662-6. doi: 10.1128/JCM.02415-08. Epub 2009 Jun 24.

Authors

Christophe Fraisier¹, Richard Stor, Bernard Tenebray, Yannick Sanson, Pierre Nicolas

Affiliation

¹ WHO Collaborating Centre, IRBA, Institut de Médecine Tropicale du Service de Santé des Armées, Marseille, France.

Abstract

We developed a new Neisseria meningitidis multiplex PCR to determine six serogroups, including X-specific primers, and to allow direct W135/Y discrimination. This assay offers a simple and low-cost method for serogrouping N. meningitidis from cerebrospinal fluid that could be useful in Africa.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Africa
Bacterial Typing Techniques / methods
Cerebrospinal Fluid / microbiology
DNA Primers / genetics
DNA, Bacterial / genetics
Genes, Bacterial
Genotype
Humans
Meningitis, Meningococcal / diagnosis*
Meningitis, Meningococcal / microbiology*
Neisseria meningitidis / classification*
Neisseria meningitidis / genetics
Neisseria meningitidis / isolation & purification*
Polymerase Chain Reaction / methods*
Serotyping / methods

Substances

DNA Primers
DNA, Bacterial