Metallo-beta-lactamase-producing Pseudomonas spp. in Korea: high prevalence of isolates with VIM-2 type and emergence of isolates with IMP-1 type

Yonsei Med J. 2009 Jun 30;50(3):335-9. doi: 10.3349/ymj.2009.50.3.335. Epub 2009 Jun 23.

Abstract

Purpose: Two Korean nationwide studies showed that metallo-beta-lactamases (MBLs)-producing-Pseudomonas spp. are not rare. The aim of this study was to assess the trends of MBL-producing isolates among imipenem-resistant isolates of Pseudomonas spp.

Materials and methods: Imipenem-resistant clinical isolates were collected from 23 hospitals and one commercial laboratory participating in the KONSAR program in 2005. Polymerase chain reaction (PCR) was used to detect MBL genes.

Results: Alleles of MBL genes were detected in 10.8% of 415 Pseudomonas aeruginosa and 66.7% of 12 P. putida isolates from 18 of 24 hospitals/laboratory. Among the 14 IMP-1-like and 39 VIM-2-like MBLs, emergence of IMP-6 was detected for the first time.

Conclusion: Prevalence of MBL-producing P. aeruginosa has not significantly increased, but IMP-6 emerged in P. aeruginosa.

Keywords: IMP-1; IMP-6; Korea; VIM-2; metallo-β-lactamase.

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Electrophoresis, Gel, Pulsed-Field
  • Humans
  • Imipenem / pharmacology
  • Korea
  • Polymerase Chain Reaction
  • Pseudomonas Infections / microbiology*
  • Pseudomonas aeruginosa / drug effects
  • Pseudomonas aeruginosa / genetics
  • Pseudomonas aeruginosa / metabolism*
  • beta-Lactamases / genetics
  • beta-Lactamases / metabolism*

Substances

  • Anti-Bacterial Agents
  • Imipenem
  • beta-lactamase IMP-1
  • beta-lactamase bla(vim-2)
  • beta-Lactamases