Genotypic analyses of Hodgkin's disease

Mol Biol Med. 1990 Dec;7(6):503-9.

Abstract

In spite of the use of molecular biology, the cellular lineage and clonality of Reed-Sternberg cells, the abnormal cells of Hodgkin's disease, remain an enigma. We studied the pattern of rearrangements at immunoglobulin and T-cell receptor loci in 23 patients suffering from Hodgkin's disease. Two out of 23 patients exhibited immunoglobulin gene rearrangements. No rearrangements of the T-cell receptor beta-chain gene were detected in any patient examined. Our results showed no correlation between the presence of rearranged bands and the number of Reed-Sternberg cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / analysis*
  • Antigens, CD / genetics
  • Blotting, Southern
  • DNA / analysis*
  • DNA Probes
  • Gene Frequency / genetics
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
  • Genotype
  • Germ Cells / chemistry
  • Hodgkin Disease / genetics*
  • Humans
  • Immunoglobulin Heavy Chains / analysis*
  • Immunohistochemistry
  • Lymph Nodes
  • Phenotype
  • Receptors, Antigen, T-Cell / analysis*

Substances

  • Antigens, CD
  • DNA Probes
  • Immunoglobulin Heavy Chains
  • Receptors, Antigen, T-Cell
  • DNA