Abstract
Two clonally related Pseudomonas aeruginosa isolates, recovered from two patients admitted to a pediatric intensive care unit, were found to harbor a new OXA-2 variant (Asn148Asp), designated OXA-161. The plasmid location of bla(OXA-161) was demonstrated through electroporation to PAO1, and its codification in a class I integron (together with aacA4) was demonstrated through PCR and sequencing. bla(OXA-2) and bla(OXA-161) were cloned in parallel to demonstrate the extended-spectrum beta-lactamase properties of OXA-161, conferring resistance to ceftazidime and reduced susceptibility to cefepime and aztreonam.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amikacin / pharmacology
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Aminoglycosides / pharmacology
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Anti-Bacterial Agents / pharmacology
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Cefepime
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Cephalosporins / pharmacology
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Clavulanic Acid / pharmacology
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Gentamicins / pharmacology
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Humans
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Imipenem / pharmacology
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Integrons / genetics*
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Meropenem
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Molecular Sequence Data
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Penicillanic Acid / analogs & derivatives
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Penicillanic Acid / pharmacology
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Piperacillin / pharmacology
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Plasmids / genetics*
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Pseudomonas Infections / microbiology*
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Pseudomonas aeruginosa / drug effects
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Pseudomonas aeruginosa / enzymology*
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Pseudomonas aeruginosa / genetics*
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Pseudomonas aeruginosa / isolation & purification
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Spanien
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Tazobactam
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Thienamycins / pharmacology
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beta-Lactamases / genetics*
Substances
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Aminoglycosides
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Anti-Bacterial Agents
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Cephalosporins
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Gentamicins
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Thienamycins
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Clavulanic Acid
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Imipenem
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Cefepime
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Amikacin
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Penicillanic Acid
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beta-Lactamases
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Meropenem
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Tazobactam
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Piperacillin
Associated data
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GENBANK/FJ617206
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GENBANK/GQ202693