The execution of the transcriptional axis mutant p53, E2F1 and ID4 promotes tumor neo-angiogenesis

Nat Struct Mol Biol. 2009 Oct;16(10):1086-93. doi: 10.1038/nsmb.1669. Epub 2009 Sep 27.

Abstract

ID4 (inhibitor of DNA binding 4) is a member of a family of proteins that function as dominant-negative regulators of basic helix-loop-helix transcription factors. Growing evidence links ID proteins to cell proliferation, differentiation and tumorigenesis. Here we identify ID4 as a transcriptional target of gain-of-function p53 mutants R175H, R273H and R280K. Depletion of mutant p53 protein severely impairs ID4 expression in proliferating tumor cells. The protein complex mutant p53-E2F1 assembles on specific regions of the ID4 promoter and positively controls ID4 expression. The ID4 protein binds to and stabilizes mRNAs encoding pro-angiogenic factors IL8 and GRO-alpha. This results in the increase of the angiogenic potential of cancer cells expressing mutant p53. These findings highlight the transcriptional axis mutant p53, E2F1 and ID4 as a still undefined molecular mechanism contributing to tumor neo-angiogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms / metabolism
  • Cell Line, Tumor
  • Cell Proliferation
  • Cytokines / metabolism
  • E2F1 Transcription Factor / genetics*
  • E2F1 Transcription Factor / metabolism
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Inhibitor of Differentiation Proteins / genetics*
  • Inhibitor of Differentiation Proteins / metabolism
  • Interleukin-8 / metabolism
  • Microcirculation
  • Mutation*
  • Neovascularization, Pathologic*
  • Transcription, Genetic*
  • Tumor Suppressor Protein p53 / genetics*
  • Tumor Suppressor Protein p53 / metabolism
  • Vascular Endothelial Growth Factor A / metabolism

Substances

  • Cytokines
  • E2F1 Transcription Factor
  • E2F1 protein, human
  • ID4 protein, human
  • Inhibitor of Differentiation Proteins
  • Interleukin-8
  • TP53 protein, human
  • Tumor Suppressor Protein p53
  • Vascular Endothelial Growth Factor A