Abstract
A whole cell biocatalytic process for uridine 5'-monophosphate (UMP) production from orotic acid by Saccharomyces cerevisiae was developed. The concentration of UMP was increased by 23% when 1 g l(-1) sodium citrate was fed into the broth. Effects of citrate addition on UMP production were investigated. Glucose-6-phosphate pool was elevated by onefold, while FBP and pyruvate were decreased by 42% and 40%, respectively. Organic acid pools such as acetate and succinate were averagely decreased by 30% and 49%. The results demonstrated that manipulation of citrate levels could be used as a novel tool to regulate the metabolic fluxes distribution among glycolysis, pentose phosphate pathway, and TCA cycle.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biotechnology / methods*
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Carbon / metabolism
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Catalysis
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Citric Acid / metabolism*
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Citric Acid / pharmacology
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Citric Acid Cycle / drug effects
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Culture Media
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Gene Expression Regulation, Enzymologic*
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Glucose / metabolism*
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Glycolysis / drug effects
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Hydrogen-Ion Concentration
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Orotic Acid / chemistry
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Orotic Acid / metabolism
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Pentose Phosphate Pathway / drug effects
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Saccharomyces cerevisiae / cytology
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins / biosynthesis
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Saccharomyces cerevisiae Proteins / metabolism
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Uridine Monophosphate / biosynthesis*
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Uridine Monophosphate / metabolism
Substances
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Culture Media
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Saccharomyces cerevisiae Proteins
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Citric Acid
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Orotic Acid
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Carbon
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Uridine Monophosphate
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Glucose