The sodium pump and cardiotonic steroids-induced signal transduction protein kinases and calcium-signaling microdomain in regulation of transporter trafficking

Biochim Biophys Acta. 2010 Dec;1802(12):1237-45. doi: 10.1016/j.bbadis.2010.01.013. Epub 2010 Feb 6.

Abstract

The Na/K-ATPase was discovered as an energy transducing ion pump. A major difference between the Na/K-ATPase and other P-type ATPases is its ability to bind a group of chemicals called cardiotonic steroids (CTS). The plant-derived CTS such as digoxin are valuable drugs for the management of cardiac diseases, whereas ouabain and marinobufagenin (MBG) have been identified as a new class of endogenous hormones. Recent studies have demonstrated that the endogenous CTS are important regulators of renal Na(+) excretion and blood pressure. The Na/K-ATPase is not only an ion pump, but also an important receptor that can transduce the ligand-like effect of CTS on intracellular protein kinases and Ca(2+) signaling. Significantly, these CTS-provoked signaling events are capable of reducing the surface expression of apical NHE3 (Na/H exchanger isoform 3) and basolateral Na/K-ATPase in renal proximal tubular cells. These findings suggest that endogenous CTS may play an important role in regulation of tubular Na(+) excretion under physiological conditions; conversely, a defect at either the receptor level (Na/K-ATPase) or receptor-effector coupling would reduce the ability of renal proximal tubular cells to excrete Na(+), thus culminating/resulting in salt-sensitive hypertension.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • Bufanolides / metabolism*
  • Calcium Signaling*
  • Humans
  • Hypertension / metabolism
  • Hypertension / pathology
  • Hypertension / physiopathology
  • Kidney Tubules, Proximal / metabolism*
  • Kidney Tubules, Proximal / pathology
  • Kidney Tubules, Proximal / physiopathology
  • Ouabain / metabolism*
  • Protein Kinases / metabolism*
  • Protein Transport
  • Sodium-Hydrogen Exchanger 3
  • Sodium-Hydrogen Exchangers / metabolism
  • Sodium-Potassium-Exchanging ATPase / metabolism*

Substances

  • Bufanolides
  • SLC9A3 protein, human
  • Sodium-Hydrogen Exchanger 3
  • Sodium-Hydrogen Exchangers
  • marinobufagenin
  • Ouabain
  • Protein Kinases
  • Sodium-Potassium-Exchanging ATPase