Aqueous extract of Trigonella foenum graecum (fenugreek) prevents cypermethrin-induced hepatotoxicity and nephrotoxicity

Hum Exp Toxicol. 2010 Apr;29(4):311-9. doi: 10.1177/0960327110361502. Epub 2010 Feb 10.

Abstract

Cypermethrin (CM) is an important type II pyrethroid pesticide used extensively in pest control and is reported to cause hepatic and renal toxicity. Oxidative stress and lipid peroxidation (LPO) has been implicated in the toxicology of pyrethroids. Fenugreek is known for its antitoxic and antioxidant potential. We have investigated the protective effect of aqueous extract of germinated fenugreek seeds in CM-induced hepatic and renal toxicity. Male Wistar rats were treated with 1/10 LD(50) (25 mg/kg body weight) of CM and 10% aqueous extract of fenugreek (GFaq) for 60 days. CM treatment caused increased thiobarbituric acid reactive substances (TBARS), depletion in glutathione (GSH) and reduction in the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) in liver and kidneys. There was a significant reduction in total phospholipids and increased activities of phospholipases A (PLA) and C (PLC) in liver and kidneys and increased activities of serum marker enzymes, aspartate transaminase (AST), alanine tansaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and gamma glutamyl transferase (GGT). Treatment with 10% GFaq showed replenishment of antioxidant status and brought all the values to near normal, indicating the protective effect of fenugreek. Phytochemicals present in fenugreek could play an important role in ameliorating the pesticide-induced toxicity.

MeSH terms

  • Animals
  • Antioxidants / metabolism
  • Antioxidants / pharmacology*
  • Biomarkers / blood
  • Biomarkers / metabolism
  • Chemical and Drug Induced Liver Injury / etiology
  • Chemical and Drug Induced Liver Injury / metabolism
  • Chemical and Drug Induced Liver Injury / prevention & control*
  • Cytoprotection
  • Disease Models, Animal
  • Enzymes / blood
  • Enzymes / metabolism
  • Glutathione / metabolism
  • Kidney / drug effects*
  • Kidney / metabolism
  • Kidney Diseases / chemically induced
  • Kidney Diseases / metabolism
  • Kidney Diseases / prevention & control*
  • Lipid Peroxidation / drug effects
  • Liver / drug effects*
  • Liver / metabolism
  • Male
  • Oxidative Stress / drug effects
  • Phospholipids / metabolism
  • Plant Extracts / pharmacology*
  • Pyrethrins
  • Rats
  • Rats, Wistar
  • Seeds
  • Thiobarbituric Acid Reactive Substances / metabolism
  • Trigonella*

Substances

  • Antioxidants
  • Biomarkers
  • Enzymes
  • Phospholipids
  • Plant Extracts
  • Pyrethrins
  • Thiobarbituric Acid Reactive Substances
  • cypermethrin
  • Glutathione