Abstract
Initial characterizations of live-Salmonella-containing early (LSEP) and late phagosomes (LSLP) in macrophages show that both phagosomes retain Rab5 and EEA1. In addition, LSEP specifically contain transferrin receptor whereas LSLP possess relatively more rabaptin-5. In contrast to LSLP, late-Salmonella-containing vacuoles in epithelial cells show significantly reduced levels of Rab5 and EEA1. Subsequent results demonstrate that both phagosomes efficiently fuse with early endosomes (EE). In contrast to LSEP, fusion between LSLP and EE is insensitive to ATPgammaS treatment. Furthermore, LSLP fuses with EE in absence of NEM-sensitive fusion factor (NSF) as well as in the presence of NSF:D1EQ mutant demonstrating that LSLP fusion with EE is NSF independent.
Copyright 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphate / metabolism
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Adenosine Triphosphate / physiology
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Cells, Cultured
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Endosomes / metabolism*
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Endosomes / pathology
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Endosomes / physiology
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Guanosine 5'-O-(3-Thiotriphosphate) / metabolism
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HeLa Cells
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Humans
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Hydrolysis
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Membrane Fusion / physiology
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Mutant Proteins / metabolism
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Mutant Proteins / physiology
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Phagocytosis / physiology
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Phagosomes / metabolism*
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Phagosomes / pathology
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Phagosomes / physiology
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Salmonella / metabolism*
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Salmonella Infections / metabolism
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Salmonella Infections / pathology
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Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins / genetics
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Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins / metabolism
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Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins / physiology*
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Time Factors
Substances
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Mutant Proteins
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Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
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Guanosine 5'-O-(3-Thiotriphosphate)
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Adenosine Triphosphate