Protein kinase C(19-31) pseudosubstrate inhibition of insulin action in rat adipocytes

FEBS Lett. 1991 Apr 22;282(1):139-42. doi: 10.1016/0014-5793(91)80463-d.

Abstract

Treatment of intact adipocytes with the autoregulatory PKC pseudosubstrate PKC(19-31) inhibited insulin-stimulated hexose uptake and lipogenesis, with no effect on basal values. The effect was dose-dependent with respective IC50 values of 30 microM and 600 microM for insulin-stimulated hexose uptake in electroporated and intact adipocytes. These studies indicate that PKC may play a role in the mediation of insulin action in adipocytes.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adipose Tissue / drug effects
  • Adipose Tissue / metabolism*
  • Amino Acid Sequence
  • Animals
  • Hexoses / metabolism
  • Insulin / physiology
  • Insulin Antagonists / pharmacology*
  • Lipids / biosynthesis
  • Molecular Sequence Data
  • Protein Kinase C / antagonists & inhibitors*
  • Rats
  • Substrate Specificity

Substances

  • Hexoses
  • Insulin
  • Insulin Antagonists
  • Lipids
  • Protein Kinase C