Interference of Mycoplasma infection in a gene expression study: it was the environment and not the gene

Appl Environ Microbiol. 2010 Dec;76(23):7867-9. doi: 10.1128/AEM.01265-10. Epub 2010 Oct 1.

Abstract

We show that short-term exposure to doxycycline, as used in tetracycline-inducible gene expression models, protects cells from stress-induced death in cultures infected with Mycoplasma arginini. Coinciding with the expected maximum level of gene activity, antimicrobial effects of tetracyclines might be mistaken for antiapoptotic properties of the expressed gene product.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Cell Line
  • Gene Expression*
  • Mice
  • Mycoplasma / drug effects*
  • Mycoplasma / pathogenicity*
  • Tetracycline / pharmacology*

Substances

  • Anti-Bacterial Agents
  • Tetracycline