CD4 intragenic SNPs associate with HIV-2 plasma viral load and CD4 count in a community-based study from Guinea-Bissau, West Africa

J Acquir Immune Defic Syndr. 2011 Jan 1;56(1):1-8. doi: 10.1097/QAI.0b013e3181f638ed.

Abstract

Objectives: The human genetics of HIV-2 infection and disease progression is understudied. Therefore, we studied the effect of variation in 2 genes that encode products critical to HIV pathogenesis and disease progression: CD4 and CD209.

Design: This cross-sectional study consisted of 143 HIV-2, 30 HIV-1 + HIV-2 and 29 HIV-1-infected subjects and 194 uninfected controls recruited from rural Guinea-Bissau.

Methods: We genotyped 14 CD4 and 4 CD209 single nucleotide polymorphisms (SNPs) that were tested for association with HIV infection, HIV-2 plasma viral load (high vs. low), and CD4 T-cell count (high vs. low).

Results: The most significant association was between a CD4 haplotype rs11575097-rs10849523 and high viral load [odds ratio (OR): = 2.37, 95% confidence interval (CI): 1.35 to 4.19, P = 0.001, corrected for multiple testing], suggesting increased genetic susceptibility to HIV-2 disease progression for individuals carrying the high-risk haplotype. Significant associations were also observed at a CD4 SNP (rs2255301) with HIV-2 infection (OR: = 2.36, 95% CI: 1.19 to 4.65, P = 0.01) and any HIV infection (OR: = 2.50, 95% CI: 1.34 to 4.69, P = 0.004).

Conclusions: Our results support a role of CD4 polymorphisms in HIV-2 infection, in agreement with recent data showing that CD4 gene variants increase risk to HIV-1 in Kenyan female sex workers. These findings indicate at least some commonality in HIV-1 and HIV-2 susceptibility.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AIDS Serodiagnosis
  • Adult
  • CD4 Antigens / genetics*
  • CD4 Antigens / immunology
  • CD4 Lymphocyte Count*
  • Cell Adhesion Molecules / genetics
  • Cell Adhesion Molecules / immunology
  • Disease Progression
  • Female
  • Genotype
  • Guinea-Bissau / epidemiology
  • HIV Infections / genetics*
  • HIV Infections / immunology
  • HIV-1 / immunology
  • HIV-2 / immunology*
  • Haplotypes
  • Humans
  • Lectins, C-Type / genetics
  • Lectins, C-Type / immunology
  • Linkage Disequilibrium / genetics
  • Logistic Models
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • Polymorphism, Single Nucleotide / genetics*
  • Polymorphism, Single Nucleotide / immunology
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / immunology
  • Viral Load / genetics*
  • Viral Load / immunology

Substances

  • CD4 Antigens
  • Cell Adhesion Molecules
  • DC-specific ICAM-3 grabbing nonintegrin
  • Lectins, C-Type
  • Receptors, Cell Surface