Background and objective: the expression of phosphatase of regenerating liver-3 (PRL-3) is correlated with Ras homologue C (RhoC) in non-small cell lung cancer (NSCLC), suggesting that they have interactions. The aim of this study is to investigate the functions of PRL-3 and RhoC in the migration of A549 cell and the potential mechanism of PRL-3 and RhoC in carcinogenesis and cancer development..
Methods: PRL-3Ab and RhoCAb were used to block the functions of PRL-3 and RhoC respectively. Wound healing assay was applied to detect the migration of A549 cell and the expression levels of PRL-3 and RhoC were detected by RT-PCR.
Results: the migration of A549 cell decreased after blockage of PRL-3 and RhoC. The expression of RhoC decreased when PRL-3 was blocked without any changes on the expression of PRL-3.
Conclusions: PRL-3, RhoC could increase cell migration in A549 cells.
背景与目的: 在前期研究中，我们发现在非小细胞肺癌中PRL-3和RhoC的表达具有相关性，提示两者可能存在相互作用。本研究通过检测两者在A549细胞迁移中的作用以及是否存在相互作用，为进一步研究它们在肿瘤发生发展中的作用机理提供实验依据。
方法: 应用PRL-3抗体、RhoC抗体分别阻断A549细胞中PRL-3和RhoC的功能，采用细胞划痕实验检测两者对其迁移能力的影响；应用RT-PCR检测PRL-3和RhoC表达的变化。
结果: PRL-3和RhoC功能被阻断后，A549细胞迁移能力明显降低；阻断PRL-3的A549细胞中RhoC表达降低，然而阻断RhoC的A549细胞中PRL-3表达无明显改变。
结论: PRL-3和RhoC可能具有促进A549细胞远处转移的功能；PRL-3可能具有上调RhoC表达的功能；PRL-3促进癌细胞远处转移的功能可能是通过RhoC及其下游因子实现的，而RhoC对PRL-3的表达可能无反馈性调节作用。