[ISSR analysis of genetic diversity in Rodgersia aesculifolia germplasm resources]

Zhong Yao Cai. 2010 Aug;33(8):1217-21.
[Article in Chinese]

Abstract

Objective: To study the genetic diversity of germplasm resources for Rodgersia aesculifolia.

Methods: 24 species of germplasm resources for Rodgersia aesculifolia were analyzed by ISSR molecular markers. To make up the systematic diagram of genetic relationship by NTSYSpc-2.11 software, clustered by UPGMA method and establish the dendrogram.

Results: A total of 109 ISSR bands was obtained by 8 primers, among which 96 were polymorphic bands. The average percentage of polymorphic bands was 88.1%. The genetic distance (GD) of the 24 Rodgersia aesculifolia accessions ranged from 0.115 to 0.877, and average 0.421. Two groups and five inferior groups were clustered by the digital data of polymorphic bands. By cluster analysis, the geographical distribution is obvious.

Conclusion: The diversity level of the different germplasm resources for Rodgersia aesculifolia higher and the relationship of Rodgersia aesculifolia correlates with the geographical location in some way.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cluster Analysis
  • DNA Primers
  • DNA, Plant / genetics
  • Genetic Variation*
  • Phylogeny
  • Plant Leaves / genetics
  • Plant Roots / genetics
  • Plants, Medicinal / classification
  • Plants, Medicinal / genetics*
  • Polymerase Chain Reaction
  • Polymorphism, Genetic
  • Repetitive Sequences, Nucleic Acid*
  • Saxifragaceae / classification
  • Saxifragaceae / genetics*

Substances

  • DNA Primers
  • DNA, Plant