Near-atomic resolution reconstructions of icosahedral viruses from electron cryo-microscopy

Nikolaus Grigorieff; Stephen C Harrison

doi:10.1016/j.sbi.2011.01.008

Near-atomic resolution reconstructions of icosahedral viruses from electron cryo-microscopy

Curr Opin Struct Biol. 2011 Apr;21(2):265-73. doi: 10.1016/j.sbi.2011.01.008. Epub 2011 Feb 18.

Authors

Nikolaus Grigorieff¹, Stephen C Harrison

Affiliation

¹ Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02154, USA.

Abstract

Nine different near-atomic resolution structures of icosahedral viruses, determined by electron cryo-microscopy and published between early 2008 and late 2010, fulfil predictions made 15 years ago that single-particle cryo-EM techniques could visualize molecular detail at 3-4Å resolution. This review summarizes technical developments, both in instrumentation and in computation, that have led to the new structures, which advance our understanding of virus assembly and cell entry.

Publication types

Research Support, N.I.H., Extramural
Review

MeSH terms

Computer Simulation*
Cryoelectron Microscopy*
Image Processing, Computer-Assisted*
Models, Molecular
Virus Assembly
Viruses / chemistry*

Abstract

Publication types

MeSH terms

Grants and funding